2019 CSCC Travelling Lectureship
Clinical Proteomics: What Every Sample Wants
Immunoassays serve as the backbone for the measurement of protein biomarkers for the care of patients. These biomarkers are used for the diagnosis, prognosis, and therapeutic management of disease. Unfortunately, many immunoassays that are used clinically can suffer from various shortcomings that lead to misleading results and improper decision-making. For example, human serum samples may include interfering substances that lead to false negative or false positive results. Additionally, results can differ dramatically between platforms, making implementation of research-driven cut-offs problematic. The Hoofnagle laboratory has spent the past decade developing liquid chromatography-tandem mass spectrometric methods to help solve the issues known to plague many immunoassays for proteins. This talk will summarize those efforts culminating with thyroglobulin, a marker of recurrent thyroid cancer, as a case study.
At the end of this session, participants will be able to:
1. List three reasons why immunoassays may generate misleading results and how mass spectrometric approaches could help provide better accuracy
2. Outline the steps of a protein assay that uses mass spectrometry as the detection method
3. Summarize how mass spectrometry can improve monitoring for thyroid cancer recurrence.
Andrew Hoofnagle, MD PhD
Andy Hoofnagle is Professor of Laboratory Medicine, Head of the Division of Clinical Chemistry, and Deputy Director of the Northwest Lipid Metabolism and Diabetes Research Laboratory, University of Washington, Seattle. His laboratory focuses on developing novel methods for the quantification of proteins and small molecules in clinical samples using LC-MS/MS. His grant-funded research continues to focus on using analytical chemistry to epidemiologically answer questions in vitamin D biology, kidney disease, cardiovascular disease, and cancer. Professor Hoofnagle mentors students, fellows, and residents in gaining a deeply respectful admiration for the power of mass spectrometry in the care of patients.
|September 20, 2019||Edmonton AB|
|September 23, 2019||Vancouver BC|
|September 25, 2019||Saskatoon SK|
|October 17, 2019||Halifax NS|
|October 18, 2019||St. John's NL|
|December 4, 2019||Montreal QC|
|December 5, 2019||Toronto ON|